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Description
The BD™ CBA Mouse Th1/Th2 Cyokine Kit (Catalog No. 551287) can be used to measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interferon-γ (IFN-γ), and Tumor Necrosis Factor (TNF) protein levels in a single sample. The kit performance has been optimized for analysis of physiologically relevant concentrations (pg/mL levels) of specific cytokine proteins in tissue culture supernatants and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples.
| Vial Label | Reagent | Quantity |
| A1 | Mouse IL-2 Capture Beads | 1 vial, 0.8 mL |
| A2 | Mouse IL-4 Capture Beads | 1 vial, 0.8 mL |
| A3 | Mouse IL-5 Capture Beads | 1 vial, 0.8 mL |
| A4 | Mouse IFN-γ Capture Beads | 1 vial, 0.8 mL |
| A5 | Mouse TNF Capture Beads | 1 vial, 0.8 mL |
| B | Mouse Th1/Th2 PE Detection Reagent | 1 vial, 4 mL |
| C | Mouse Th1/Th2 Cytokine Standards | 2 vials lyophilized |
| D | Cytometer Setup Beads | 1 vial, 1.5 mL |
| E | PE Positive Control Detector | 1 vial, 0.5 mL |
| E2 | FITC Positive Control Detector | 1 vial, 0.5 mL |
| F | Wash Buffer | 1 bottle, 130 mL |
| G | Assay Diluent | 1 bottle, 30 mL |
Components
| Description | Quantity/Size | Part Number |
| Mouse IL-2 Capture Beads | N/A | 51-9005801 |
| Mouse IL-4 Capture Beads | N/A | 51-9005803 |
| Mouse IL-5 Capture Beads | N/A | 51-9005805 |
| Mouse IFN-γ Capture Beads | N/A | 51-9005807 |
| Mouse TNF Capture Beads | N/A | 51-9005809 |
| B) Mouse Th1/Th2 PE Detection Reagent | N/A | 51-2523KC |
| Mouse Th1/Th2 Cytokine Standards | N/A | 51-2524KC |
| D Cytometer Setup Beads | 1 Each (1 ea) | 51-9005731 |
| E1 PE Positive Control Detector | 1 Each (1 ea) | 51-9005732 |
| E2 FITC Positive Control Detector | 1 Each (1 ea) | 51-9005734 |
| F Wash Buffer | 1 Each (1 ea) | 51-2431KC |
| G Assay Diluent | 1 Each (1 ea) | 51-2432KC |
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