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After the primary antibody binds to the target protein, it forms a complex with the HRP-conjugated secondary antibody. Add LumiGLO*, which emits light during enzyme-catalyzed breakdown.
Application Keywords:
WB - Western blot IP -immunoprecipitation IHC - Immunohistochemistry ChIP -chromatin immunoprecipitation IF - Immunofluorescence F - Flow Cytometry EP -ELISA Peptides
Species cross-reactivity keywords:
H - person M -mouse R - rat Hm - Hamster Mk - Monkey Vir - Virus Mi - Mink C - Chicken Dm - Drosophila melanogaster X - Xenopus Z -zebrafish B - cattle DG - Canine PG - Pig Sc - Saccharomyces cerevisiae Ce - Caenorhabditis elegans Hr - Horse All - All species are expected
Recommended antibody dilutions:
1:1000–1:3000
20X LumiGLO® Reagent and 20X Peroxide #7003 1:1K–1:3K
SignalFireTM ECL Reagent #6883 1:1K–1:3K
SignalFireTM Plus ECL Reagent #12630 1:5K-1:15K
SignalFireTM Elite ECL Reagent #12757 1:10K-1:20K
Stored in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA), 50% glycerol. Store at -20°C. Do not aliquot the antibody. This product is stable for 36 months at -20C.