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After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Recommended Antibody Dilutions:
1:1000–1:3000
20X LumiGLO® Reagent and 20X Peroxide #7003 1:1K–1:3K
SignalFireTM ECL Reagent #6883 1:1K–1:3K
SignalFireTM Plus ECL Reagent #12630 1:5K-1:15K
SignalFireTM Elite ECL Reagent #12757 1:10K-1:20K
Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. This product is stable for 36 months when stored at -20C.