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首页 > 产品中心 > 抗体 > 一抗 > PD-L1 (E1L3N ® ) XP Rabbit mAb

PD-L1 (E1L3N ® ) XP Rabbit mAb

简要描述:抗体名: PD-L1(E1L3N) ) XP兔单克隆抗体 浓度: 见优宁维 % 靶点: 见优宁维 宿主: Rabbit 用途范围: 详见说明书 ......

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  • 更新时间:2022-07-13
  • 访  问  量:982
详细介绍

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反应性 H
灵敏度 内源性
MW (kDa) 40-50
来源/同种型 兔 IgG

应用关键词:

  • WB- 蛋白质印迹法
  • IP-免疫沉淀法
  • IHC-免疫组织化学法
  • ChIP-染色质免疫沉淀法
  • IF-免疫荧光法
  • F-流式细胞术
  • E-P-ELISA 肽

物种交叉反应性关键词:

  • H-人
  • M-小鼠
  • R-大鼠
  • Hm- 仓鼠
  • Mk-猴
  • Vir- 病毒
  • Mi-水貂
  • C-鸡
  • Dm-黑腹果蝇
  • X-爪蟾
  • Z-斑马鱼
  • B-牛
  • DG-犬
  • PG-猪
  • Sc-酿酒酵母
  • Ce-秀丽隐杆线虫
  • Hr-马
  • All-预期所有物种

产品使用信息

应用 稀释度
蛋白质印迹法 1:1000
免疫沉淀法 1:50
IHC-Leica® Bond™ 1:200 - 1:800
免疫组织化学(石蜡) 1:100 - 1:400
流式细胞术 1:200 - 1:800

保存

保存在 10 mM sodium HEPES (pH 7.5)、150 mM NaCl、100 μg/ml BSA、50% 甘油和低于 0.02% 的中。-20℃ 保存。切勿分装抗体。

特异性/灵敏度

PD-L1 (E1L3N®) XP® Rabbit mAb 可识别内源水平的 PD-L1 总蛋白。

物种反应性:

来源/纯化

使用与人 PD-L1 蛋白中羧基末端周围的残基相对应的合成肽,对动物进行免疫接种来产生单克隆抗体。

背景

Programmed cell death protein 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds to the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered after many B7 protein homologues were found, and it was later shown to be expressed in antigen-presenting cells, activated T cells, and tissues such as placenta, heart, and lung (1-3). PD-L1 is structurally similar to B7 family members in that it contains extracellular IgV and IgC domains and a short cytoplasmic region. Studies have shown that PD-L1 is expressed in cells of many tumor types including melanoma, ovarian, colon, lung, breast and renal cell carcinomas (4-6). PD-L1 expression in cancer cells is associated with tumor-infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Other studies have implicated PD-L1 expression in viral infection-related cancers (8,9).
  1. Dong, H. et al. (1999) Nat Med 5, 1365-9.
  2. Freeman, GJ et al. (2000) J Exp Med 192, 1027-34.
  3. Liang, SC et al. (2003) Eur J Immunol 33, 2706-16.
  4. Dong, H. et al. (2002) Nat Med 8, 793-800.
  5. Thompson, RH et al. (2006) Cancer Res 66, 3381-5.
  6. Pardoll, DM (2012) Nat Rev Cancer 12, 252-64.
  7. Taube, JM et al. (2012) Sci Transl Med 4, 127ra37.
  8. Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41.
  9. Chen, BJ et al. (2013) Clin Cancer Res 19, 3462-73.
  10. Wimberly, H. et al. (2014) Cancer Immunol Res ,


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