RnDsystems 2D3D细胞培养解决方案

更新时间：2017-03-28

阅读：764

细胞粘附 | 细胞侵袭 | 细胞迁移 | 3-D培养

基底膜（Basement Membrane, BM）是特定细胞外基质，在内皮细胞、上皮细胞、肌肉或神经细胞间形成界面；BM不仅可以支持细胞层，还在组织形成中影响细胞粘附、迁移、增殖和分化

3-D细胞培养是一种人工建立的环境，细胞可以在三维层面上在该系统生长并与周围微环境相互作用；在3-D模型中，特定的细胞和基质环境提供模拟组织的环境，在药物筛选、肿瘤和干细胞研究中具有重要意义

Cultrex Basement Membrane Extract — 的基质胶/基底膜提取物提取于Engelbreth-Holm-Swarm (EHS)小鼠肉瘤，在37°C重新形成基底膜；其主要成分是laminin, collagen IV, entactin, HSPG等；主要用于支持细胞生长和分化，也用于细胞粘附、血管新生、提体外细胞迁移/侵袭和体内成瘤实验等；
产品批间一致性与竞争产品相比， Cultrex BME的批间一致性 R: reduced growth factor, N: non-reduced growth factor		污染物的控制和检测 • 内毒素< 8 EU/ml • 无细菌/真菌污染（依据USP XXIV第71章，37°C培养14天检测） • 无支原体污染（PCR污染） • PathClear系列对支原体和30种其他病原体/病毒检测

Cultrex BME产品种类和应用范围

NAME	BUFFER	CONCENTRATION	RECOMMENDED APPLICATIONS
Cultrex® Original BME	DMEM	12-18 mg/ml	General (2D Culture, 3-D Culture, Attachment, Invasion,etc.)
Cultrex® Stem Cell Qualified BME	DMEM	12-18 mg/ml	Attachment and Maintenance of Stem Cells
Cultrex® 3-D ulture Matrix™ ME	DMEM	14-16 mg/ml	3-D Culture
Cultrex® Organoid Qualified BME, Type 2	DMEM	12-18 mg/ml	Robust Organoid Culture
Cultrex® BME, Type 3	RPMI1640	12-18 mg/ml	Xenograft/Tumorgraft
Cultrex® Organoid ualified BME, Type R1	DMEM	12-18 mg/ml	Difficult to Grow Organoid Culture

其他蛋白产品
	• Cultrex Mouse Laminin I • Cultrex Collagen I (Bovine) • Cultrex Rat Collagen I • Cultrex Mouse Collagen IV • Cultrex 3-D Culture Matrix Laminin I • Cultrex 3-D Culture Matrix Rat Collagen I • Cultrex Human Vitronectin • Cultrex Human Fibronectin		• Cultrex Mouse Laminin I • Cultrex Fibronectin (Bovine) • Cultrex Vitronectin (Bovine) • Cultrex Poly-D-Lysine • Cultrex Poly-D-Lysine • Cultrex Spheroid Formation Matrix • Cultrex Spheroid Invasion Matrix

肿瘤相关培养研究		细胞侵袭实验

细胞迁移实验

细胞迁移实验用以定量细胞应答趋化因子和/或抑制刺激化合物的迁移能力

Description	Size	Catalog No
Cultrex 24 Well Cell Migration Assay	24 Samples	3465-024-K
Cultrex 96 Well Cell Migration Assay	24 Samples	3465-096-K

细胞粘附实验

CultreCoat® 细胞粘附实验是一种简单、标准化、高通量的评估影响细胞基质相互作用因子的工具

Description	Size	Catalog No
CultreCoatAdhesion Protein Array Kit	96 Wells	3496-096-K
CultreCoatBME 96 Well Cell Adhesion Assay	96 Wells	3490-096-K
CultreCoatCollagen I 96 Well Cell Adhesion Assay	96 Wells	3492-096-K
CultreCoatCollagen IV 96 Well Cell Adhesion Assay	96 Wells	3465-096-K
CultreCoatLaminin I 96 Well Cell Adhesion Assay	96 Wells	3465-024-K
CultreCoatVitronectin96 Well Cell Adhesion Assay	96 Wells	3465-096-K
CultreCoatFibronectin 96 Well Cell Adhesion Assay	96 Wells	3465-024-K

内皮细胞侵袭实验

筛选影响血管内皮细胞侵袭的化合物或因子

Description	Size	Catalog No
Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit	96 Tests	3471-096-K

3-D 培养

3-D培养可以提供细胞必要的结构和信号诱因来介导多细胞结构的重塑；该方法提供体外模拟生理环境，用以评估发育和疾病

Description	Size	Catalog No
Cultrex 3-D Culture Matrix Reduced Growth Factor Basement Membrane Extract, PathClear	1ml 5ml 2*5ml	3445-001-01 3445-005-01 3445-010-01
Cultrex Organoid Qualified Basement Membrane Extract (type 2), PathClear	1ml 5ml 2*5ml	3532-001-02 3532-005-02 3532-010-02
Cultrex Organoid Qualified, Reduced Growth Factor Basement Membrane Extract (type 2), PathClear	1ml 5ml 2*5ml	3533-001-02 3533-005-02 3533-010-02
Cultrex 3-D Culture Matrix Laminin I	30mg	3446-005-01
Cultrex 3-D Culture Matrix Rat Collagen I	20ml	3447-020-01
Cultrex 3-D Spheroid Cell Invasion Assay	96 Samples	3500-096-K
Cultrex 3-D Spheroid FluorometricProliferation/Viability Assay	96 Samples	3510-096-K
Cultrex 3-D Spheroid Colorimetric Proliferation/Viability Assay	96 Samples	3511-096-K