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首页 > 新闻中心 > RnDsystems 2D3D细胞培养解决方案

RnDsystems 2D3D细胞培养解决方案

更新时间:2017-03-28

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                                                   细胞粘附 | 细胞侵袭 | 细胞迁移 | 3-D培养

 

      基底膜(Basement Membrane, BM)是特定细胞外基质,在内皮细胞、上皮细胞、肌肉或神经细胞间形成界面;BM不仅可以支持细胞层,还在组织形成中影响细胞粘附、迁移、增殖和分化
      3-D细胞培养是一种人工建立的环境,细胞可以在三维层面上在该系统生长并与周围微环境相互作用;在3-D模型中,特定的细胞和基质环境提供模拟组织的环境,在药物筛选、肿瘤和干细胞研究中具有重要意义

Cultrex Basement Membrane Extract — 的基质胶/基底膜提取物
      提取于Engelbreth-Holm-Swarm (EHS)小鼠肉瘤,在37°C重新形成基底膜;其主要成分是laminin, collagen IV, entactin, HSPG等;主要用于支持细胞生长和分化,也用于细胞粘附、血管新生、提体外细胞 迁移/侵袭和体内成瘤实验等;

产品批间一致性
与竞争产品相比, Cultrex BME的 批间一致性
R: reduced growth factor,
N: non-reduced growth factor

污染物的控制和检测
• 内毒素< 8 EU/ml
• 无细菌/真菌污染( 依据USP XXIV第71章,37°C培养14天检测)
• 无支原体污染(PCR污染)
• PathClear系列对支原体和30种其他病原体/病毒检测
Cultrex BME产品种类和应用范围
NAMEBUFFERCONCENTRATIONRECOMMENDED APPLICATIONS
Cultrex® Original BMEDMEM12-18 mg/mlGeneral (2D Culture, 3-D Culture, Attachment, Invasion,etc.)
Cultrex® Stem Cell Qualified BMEDMEM12-18 mg/mlAttachment and Maintenance of Stem Cells
Cultrex® 3-D ulture Matrix™ MEDMEM14-16 mg/ml3-D Culture
Cultrex® Organoid Qualified BME, Type 2DMEM12-18 mg/mlRobust Organoid Culture
Cultrex® BME, Type 3RPMI164012-18 mg/mlXenograft/Tumorgraft
Cultrex® Organoid ualified BME, Type R1DMEM12-18 mg/mlDifficult to Grow Organoid Culture
其他蛋白产品
 • Cultrex Mouse Laminin I
• Cultrex Collagen I (Bovine)
• Cultrex Rat Collagen I
• Cultrex Mouse Collagen IV
• Cultrex 3-D Culture Matrix Laminin I
• Cultrex 3-D Culture Matrix Rat Collagen I
• Cultrex Human Vitronectin
• Cultrex Human Fibronectin
 • Cultrex Mouse Laminin I
• Cultrex Fibronectin (Bovine)
• Cultrex Vitronectin (Bovine)
• Cultrex Poly-D-Lysine
• Cultrex Poly-D-Lysine
• Cultrex Spheroid Formation Matrix
• Cultrex Spheroid Invasion Matrix

 

肿瘤相关培养研究 细胞侵袭实验
细胞迁移实验
细胞迁移实验用以定量细胞应答趋化因子和/或抑制刺激化合物的迁移能力 
DescriptionSizeCatalog No
Cultrex 24 Well Cell Migration Assay24 Samples3465-024-K
Cultrex 96 Well Cell Migration Assay24 Samples3465-096-K

 

细胞粘附实验
CultreCoat® 细胞粘附实验是一种简单、标准化、高通量的评估影响细胞基质相互作用因子的工具
DescriptionSizeCatalog No
CultreCoatAdhesion Protein Array Kit96 Wells3496-096-K
CultreCoatBME 96 Well Cell Adhesion Assay96 Wells3490-096-K
CultreCoatCollagen I 96 Well Cell Adhesion Assay96 Wells3492-096-K
CultreCoatCollagen IV 96 Well Cell Adhesion Assay96 Wells3465-096-K
CultreCoatLaminin I 96 Well Cell Adhesion Assay96 Wells3465-024-K
CultreCoatVitronectin96 Well Cell Adhesion Assay96 Wells3465-096-K
CultreCoatFibronectin 96 Well Cell Adhesion Assay96 Wells3465-024-K

 

内皮细胞侵袭实验
筛选影响血管内皮细胞侵袭的化合物或因子 
DescriptionSizeCatalog No
Cultrex In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit96 Tests3471-096-K
3-D 培养
3-D培养可以提供细胞必要的结构和信号诱因来介导多细胞结构的重塑;该方法提供体外模拟生理环境,用以评估发育和疾病 
DescriptionSizeCatalog No
Cultrex 3-D Culture Matrix Reduced Growth Factor Basement Membrane Extract, PathClear1ml
5ml
2*5ml
3445-001-01
3445-005-01
3445-010-01
Cultrex Organoid Qualified Basement Membrane Extract (type 2), PathClear1ml
5ml
2*5ml
3532-001-02
3532-005-02
3532-010-02
Cultrex Organoid Qualified, Reduced Growth Factor Basement Membrane Extract (type 2), PathClear1ml
5ml
2*5ml
3533-001-02
3533-005-02
3533-010-02
Cultrex 3-D Culture Matrix Laminin I30mg3446-005-01
Cultrex 3-D Culture Matrix Rat Collagen I20ml3447-020-01
Cultrex 3-D Spheroid Cell Invasion Assay96 Samples3500-096-K
Cultrex 3-D Spheroid FluorometricProliferation/Viability Assay96 Samples3510-096-K
Cultrex 3-D Spheroid Colorimetric Proliferation/Viability Assay96 Samples3511-096-K