研究活细胞中蛋白定位和动力学的利器—Chromobody® 质粒和抗体

在研究活细胞中蛋白定位和动力学的过程中是不是会有如下的困惑：

1.    固定样品中细胞表达的荧光融合蛋白信号强度在生理表达水平通常很低？
2.    荧光蛋白的耐光性和量子效率达不到超分辨显微镜的要求（如：3D-SIM或者STED）？
3.    许多细胞生物学方法如Hcl处理的BrdU检测，EdU-Click-It处理或者热变性的荧光原位杂交导致荧光蛋白的信号干扰？
 
    ChromoTek公司的Booters选自于ATTO公司的超荧光染料共价结合在小片段高特异性的GFP, RFP结合蛋白上面，使用ChromoTek公司的增强剂达到如下目的：重新激活，增强荧光信号，使融合蛋白的信号更稳定。

详情请咨询*代理商：上海优宁维生物科技有限公司。
 

 
产品的应用及应用范围如下：

 
产品信息如下：

 
 
ChromoTek公司的Chromobodies^®是一种新的极其微小的细胞内功能抗体。它是基于融合有荧光蛋白(e.g.TagGFP or TagRFPfromEvrogen)的来自养骆的抗体重链的抗原结合域结构(V_HH)。和普通抗体不同的是，这些创新的荧光纳米探针可以使活细胞的结构和进程实时分析和可视化。因此，Chromobodies^® 是做研究细胞内研究包含高含量分析（HCA）的一种非常的试剂。
除了稳定细胞株，你也可以得到Chromobody®质粒，它允许你创建自己的细胞系或瞬时表达Chromobody®。
所有Chromobody®结合域都是经过精心挑选，以不干扰内源性蛋白质的功能，并提供了*的机会来标记活细胞中天然蛋白。
 

产品有：

 
 
部分用户反馈：
“Your GFP-Trap^® is great! I have never seen such efficient reagent for pull down.”
“We were gob smacked when we did our own IP and saw the glowing green GFP-Trap® beads..”
“I am brim over with enthusiasm for your GFP-Trap®;great product and great idea; Hats off!”
“We have been pleasedwith the results from both of the products: GFP-Trap_A® andGFP-Trap_M®”.
“The beads GFP-Trap®_Mare fantastic!”
"...the beads were glowing purple so we knew there was a ton of mCherry on them! I think the RFP-Trap® is a really nice reagent..."
"We have found your trap tools extremely useful, the purification effect is way superior to most of our antibodies. This tool proved very helpful in our research and we are practically addicted on it."
"I tested the RFP-Booster for labeling Drosophila oocytes using the suggested protocol. The Booster was specific and had double the fluorescence intensity as the fixed mCherry control. Even more exciting was that the dye was wonderfully stable. We imaged 9000 frames and had very little (if any) bleaching."
"The 5F8 antibody is great. We have beautiful staining in the olfactory bulb for our neurons electroporated with RFP-encoding vector"
 
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