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首页 > 技术文章 > BioXcell:InVivoMAb anti-mouse F4/80

BioXcell:InVivoMAb anti-mouse F4/80

更新时间:2022-08-02

阅读:368

一 、产品介绍
F4/80是一种160 kDa的膜糖蛋白,属于EGF-TM7家族。F4/80在大多数组织巨噬细胞上表达,包括腹膜、肺、肠、胸腺和脾红髓巨噬细胞,以及Kuffer细胞、朗格汉斯细胞、骨髓基质细胞和树突细胞的亚群。F4/80的同源配体尚未确定,但在小鼠中的研究表明,F4/80对细胞因子的产生和诱导调节性T细胞是重要的。



二 、产品详情

产品详情
产品货号BE0206
产品规格1/5/25/50/100mg
抗体亚型Rat IgG2b, κ
推荐同型对照InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin(货号:BE0090
推荐抗体稀释液InVivoPure™ pH 7.0 Dilution Buffer(货号:IP0070
免疫原C57BL/6 mouse thioglycollate stimulated peritoneal macrophages
应用in vivo Monocyte/Macrophage depletion/Functional assays/Immunohistochemistry (paraffin)/Immunohistochemistry (frozen)/Flow cytometry
产品形式PBS , pH 7.0   Contains no stabilizers or preservatives
内毒素水平<2EU/mg (<0.002EU/μg)使用 LAL gel clotting 测定
纯度>95% Determined by SDS-PAGE
无菌处理0.2 μM filtered
生产形式从组织培养上清液中纯化得到。
纯化形式Protein G
RRIDAB_10949019
分子量大小150 kDa
保存条件抗体原溶液应保存在4°C条件下,不要冷冻保存。


三 、已发表文献

用途已发表文献
in vivo Monocyte/Macrophage depletionPaul, S., et al. (2019). “Natural killer T cell activation increases iNOS(+)CD206(-) M1 macrophage and controls the growth of solid tumor." J Immunother Cancer 7(1): 208
in vivo Monocyte/Macrophage depletionWang, E. C. E., et al. (2019). “A Subset of TREM2(+) Dermal Macrophages Secretes Oncostatin M to Maintain Hair Follicle Stem Cell Quiescence and Inhibit Hair Growth." Cell Stem Cell 24(4): 654-669.e656
in vivo Monocyte/Macrophage depletionWang, W., et al. (2018). “RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer." Cancer Cell 34(5): 757-774 e757
in vivo Monocyte/Macrophage depletionAlbacker, L. A., et al. (2013). “TIM-4, expressed by medullary macrophages, regulates respiratory tolerance by mediating phagocytosis of antigen-specific T cells." Mucosal Immunol 6(3): 580-590
Flow cytometryTittel, A. P., et al. (2012). “Functionally relevant neutrophilia in CD11c diphtheria toxin receptor transgenic mice." Nat Methods 9(4): 385-390.
Flow cytometryWinkler, I. G., et al. (2012). “Hematopoietic stem cell mobilizing agents G-CSF, cyclophosphamide or AMD3100 have distinct mechanisms of action on bone marrow HSC niches and bone formation." Leukemia 26(7): 1594-1601
in vivo Monocyte/Macrophage depletionBedoret, D., et al. (2009). “Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice." J Clin Invest 119(12): 3723-3738.
Immunohistochemistry (paraffin)Joffre, O., et al. (2008). “Prevention of acute and chronic allograft rejection with CD4+CD25+Foxp3+ regulatory T lymphocytes." Nat Med 14(1): 88-92.
Immunohistochemistry (paraffin)Lloyd, C. M., et al. (2008). “Three-colour fluorescence immunohistochemistry reveals the diversity of cells staining for macrophage markers in murine spleen and liver." J Immunol Methods 334(1-2): 70-81
Immunohistochemistry (paraffin)Pull, S. L., et al. (2005). “Activated macrophages are an adaptive element of the colonic epithelial progenitor niche necessary for regenerative responses to injury." Proc Natl Acad Sci U S A 102(1): 99-104
Functional assaysWarschkau, H. and A. F. Kiderlen (1999). “A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes." J Immunol 163(6): 3409-3416


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